Anti-GFP Tag Antibody

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Anti-GFP Tag Antibody (Rabbit Monoclonal 抗体) 产品信息

产品名称
Anti-GFP Tag Antibody
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Aequorea victoria
特异性
Aequorea victoria GFP Tag
免疫原
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
来源
Monoclonal Rabbit IgG Clone #208
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GFP Tag Antibody (Rabbit Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1/1000-1/5000
ELISA 1/10000-1/20000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GFP Tag Antibody (Rabbit Monoclonal 抗体) 图片

Anti-GFP Tag was immunoprecipitated using:

Lane A: GFP transfected E.coli cell lysate (0.5mg)

Lane B: GFP transfected 293 cell lysate (0.5mg)

 

Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GFP rabbit monoclonal antibody at 1:500 dilution

Lane A: GFP transfected E.coli lysate (5ug)

Lane B: GFP transfected 293 Cell lysate (30ug)

Lane C: Non-transfected 293 Cells lysate (30ug)

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

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