Anti-GST tag Antibody

Anti-GST tag Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-GST tag Antibody
经验证的应用
WB,IP
交叉反应
Reacts with: Schistosoma japonicum
特异性
Schistosoma japonicum GST tag
No cross-reactivity with E.coli cell lysate in ELISA.
免疫原
Recombinant GST protein
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #6C10G4
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GST tag Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1/1000-1/10000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GST tag Antibody (Mouse Monoclonal 抗体) 图片

Anti-GST Tag was immunoprecipitated using:

Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)

Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)

 

2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human ERK2.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)

Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human USP7.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)

Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by 293 cell.

Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500

Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)

Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)

Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)

Lane D: Negative control

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by E. coli.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250

Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)

Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)

Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)

Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)

Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)

Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)

Lane K: Empty vecter (0.5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000

Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane C: Empty vecter (0.1ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000

Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane C: Empty vecter (0.2ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

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