GST (蛋白 | 抗体 | cDNA 克隆 | ELISA 试剂盒)

All GST reagents are produced in house and quality controlled, including 1 GST Lysate, 2 GST Protein. All GST reagents are ready to use.

GST 相关研究领域

GST 分子背景

Genetic engineershave used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into anexpression vectoralongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to createfusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like themyc- or theFLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.

GST 参考文献

    • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
    • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
    • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
    • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.