CD83 ELISA Kit, Human

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CD83 ELISA Kit, Human: 产品信息

产品名称
CD83 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
7.47 pg/mL
线性范围
7.81-500 pg/mL
特异性
Recognizes both recombinant and natural Human CD83
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 61.64 136.43 299.12
SD 2.13 5.45 15.70
CV(%) 3.5% 4.0% 5.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 67.47 135.06 264.44
SD 4.39 5.18 6.66
CV(%) 6.5% 3.8% 2.5%
回收率
The recovery of Human CD83 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
92
Range
92-93%
线性关系
Supernatant
  Recovery of detected
1:2 103%
1:4 97%
1:8 93%
1:16 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD83 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD83 protein in Lysate . It contains recombinant Human CD83, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD83 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD83. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD83 ELISA Kit, Human: 别称

BL11 ELISA Kit, Human; HB15 ELISA Kit, Human

CD83 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD83 is considered as a marker of mature dendritic cells as well as an adhesion receptor that binds to resting monocytes and a subset of activated CD8+ T cells. In certain conditions, CD83 tended to dimerize or even multimerize through its aberrant intermolecular disulfide bonds. The injection of CD83-Ig can significantly enhaunce the rate of tumor growth and inhibit the T cell growth.
全称
CD83 molecule
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
  • Scholler N, et al.(2002) Cutting Edge: CD83 Regulates the Development of Cellular Immunity. The Journal of Immunology. 168 (6): 2599-602.
  • Lechmann M, et al.(2002) CD83 on dendritic cells: more than just a marker for maturation. Trends in immunology. 23(6): 273-5.
  • CD83 is a new potential biomarker and therapeutic target for Hodgkin lymphoma
    Author
    Li, Z;Ju, X;Lee, K;Clarke, C;Hsu, JL;Abadir, E;Bryant, CE;Pears, S;Sunderland, N;Heffernan, S;Hennessy, A;Lo, TH;Pietersz, GA;Kupresanin, F;Fromm, PD;Silveira, PA;Tsonis, C;Cooper, WA;Cunningham, I;Brown, C;Clark, GJ;Hart, DNJ;,
    Year
    2018
    Journal
    Haematologica
    Application
    ELISA
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