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人 CSF1R / MCSF Receptor / CD115 ELISA Kit

  • Human CSF1R / MCSF Receptor / CD115 ELISA Kit, TYPICAL DATA
  • Human CSF1R / MCSF Receptor / CD115 ELISA Kit, SPECIFICITY
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检测原理
Solid Phase Sandwich ELISA
预期用途
For the quantitative determination of Human CSF1R / MCSF Receptor / CD115 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
线性范围
31.25-2000 pg/mL
灵敏度
The minimum detectable dose of Human CSF1R / MCSF Receptor / CD115 is typically less than 7.95 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
试剂盒组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
储存方法
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
 CSF1R ELISA 检测试剂盒标准曲线
Human CSF1R / MCSF Receptor / CD115 ELISA Kit, TYPICAL DATA
[点击查看大图]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
 CSF1R ELISA 检测试剂盒特异性
Human CSF1R / MCSF Receptor / CD115 ELISA Kit, SPECIFICITY
[点击查看大图]
Caption:
This assay recognizes both recombinant and natural Human MCSFR. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Recombinant human CSF1 prepared at 50 ng/mL were assayed and exhibited no interference.

MCSF Receptor/CSF1R/CD115 研究背景

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

 MCSF Receptor/CSF1R/CD115 参考资料
  • Sherr CJ. (1990) The colony-stimulating factor 1 receptor: pleiotropy of signal-response coupling. Lymphokine Res. 9(4): 543-8.
  • Kacinski BM. (1997) CSF-1 and its receptor in breast carcinomas and neoplasms of the female reproductive tract. Mol Reprod Dev. 46(1): 71-4.
  • Sapi E, et al. (1999) The role of CSF-1 in normal and neoplastic breast physiology. Proc Soc Exp Biol Med. 220(1): 1-8.
  • Sapi E. (2004) The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update. Exp Biol Med (Maywood). 229(1): 1-11.
  • Bonifer C, et al. (2008) The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis. Front Biosci. 13: 549-60.
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    货号: KIT10161-1
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