Isocitrate dehydrogenase / IDH1 cDNA ORF Clone, Human, N-GFPSpark® tag

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Isocitrate dehydrogenase / IDH1 cDNA ORF Clone, Human, N-GFPSpark® tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1245 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human isocitrate dehydrogenase 1 (NADP+), soluble with N terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.98kb)
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Isocitrate dehydrogenase / IDH1 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Isocitrate dehydrogenase / IDH1 cDNA ORF Clone, Human, N-GFPSpark® tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

Isocitrate dehydrogenase / IDH1 cDNA ORF Clone, Human, N-GFPSpark® tag: 别称

HEL-216 cDNA ORF Clone, Human; HEL-S-26 cDNA ORF Clone, Human; IDCD cDNA ORF Clone, Human; IDH cDNA ORF Clone, Human; IDP cDNA ORF Clone, Human; IDPC cDNA ORF Clone, Human; PICD cDNA ORF Clone, Human

Isocitrate dehydrogenase / IDH1 背景信息

全称
isocitrate dehydrogenase 1 (NADP+), soluble
参考文献
  • Takano S, et al. (2011) Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing. Brain Tumor Pathol. 28(2): 115-23.
  • Geisbrecht BV, et al. (1999) The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J Biol Chem. 274(43): 30527-30533.
  • Nekrutenko A, et al. (1998) Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family. Mol Biol Evol. 15(12): 1674-1684.
  • Henke B, et al. (1998) IDP3 encodes a peroxisomal NADP-dependent isocitrate dehydrogenase required for the beta-oxidation of unsaturated fatty acids. J Biol Chem. 273(6): 3702-3711.
  • Gabriel JL, et al. (1986) Activity of purified NAD-specific isocitrate dehydrogenase at modulator and substrate concentrations approximating conditions in mitochondria. Metabolism. 35(7): 661-667.
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