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人 Interferon Gamma / IFN gamma / IFNG ELISA Kit

  • Human Interferon Gamma/IFN gamma/IFNG ELISA Kit Standard Curve 17955
  • Human Interferon Gamma/IFN gamma/IFNG ELISA Kit Specificity 17956
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检测原理
Solid Phase Sandwich ELISA
预期用途
For the quantitative determination of Human Interferon Gamma / IFN gamma / IFNG concentration in cell culture supernates.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
线性范围
46.88-3000 pg/mL
灵敏度
The minimum detectable dose of Human Interferon Gamma / IFN gamma / IFNG is typically less than 28.92 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
试剂盒组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
储存方法
Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05T), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP
Interferon Gamma/IFN gamma/IFNG 研究背景

IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

 Interferon Gamma/IFN gamma/IFNG 参考资料
  • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
  • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
  • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
  • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
  • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
  • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
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    货号: KIT11725-1
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