MTOR cDNA ORF Clone, Human, C-OFPSpark® tag

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MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
7650 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human mechanistic target of rapamycin (serine/threonine kinase) with C terminal OFPSpark / RFP tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
HindIII + NotI (6kb + 8.34kb)
标签序列
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

MTOR cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: 验证图片

MTOR cDNA ORF Clone, Human, C-OFPSpark® tag: 别称

FRAP cDNA ORF Clone, Human; FRAP1 cDNA ORF Clone, Human; FRAP2 cDNA ORF Clone, Human; RAFT1 cDNA ORF Clone, Human; RAPT1 cDNA ORF Clone, Human; SKS cDNA ORF Clone, Human

MTOR 背景信息

The protein encoded by MTOR gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
全称
mechanistic target of rapamycin (serine/threonine kinase)
参考文献
  • Zhao J, Zhai B, Gygi SP, Goldberg AL. mTOR inhibition activates overall protein degradation by the ubiquitin proteasome system as well as by autophagy. Proceedings of the National Academy of Sciences of the United States of America. 2015;112(52):15790-15797.
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