ICAM-1 Protein, Mouse, Recombinant (His Tag)

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ICAM-1 Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 96 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 40% cells will adhere specifically.
蛋白构建
A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Mouse
预测 N 端
Gln 28
分子量
The recombinant mouse ICAM1 consists of 469 amino acids after removal of the signal peptide. and has a predicted molecular mass of 51.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1 is approximately 80-90 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ICAM-1 Protein, Mouse, Recombinant (His Tag): 图片

ICAM-1 Protein, Mouse, Recombinant (His Tag): 别称

CD54 Protein, Mouse; Icam-1 Protein, Mouse; Ly-47 Protein, Mouse; MALA-2 Protein, Mouse

ICAM-1 背景信息

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
全称
intercellular adhesion molecule 1
研究领域
参考文献
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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