IGF1 Protein, Human, Recombinant

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IGF1 Protein, Human, Recombinant: 产品信息

纯度
> 95 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA.
2. Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA.
3. Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA.
4. Measured by its ability to bind human IGF1R (aa 954-1367)-GST (Cat:10164-H20B1) in a functional ELISA.
5. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2 ng/mL.
6. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 3.5-14 ng/mL.
蛋白构建
A DNA sequence encoding the mature form of human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) was expressed and purified.
NCBI Reference No.
表达宿主
E. coli
种属
Human
预测 N 端
Met
分子量
The recombinant mature form of human IGF1 consisting of 71 amino acids and has a calculated molecular mass of 7.8KDa.
缓冲液
Lyophilized from sterile 30% Acetonitrile, 0.1% TFA
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IGF1 Protein, Human, Recombinant: 图片

IGF1 Protein, Human, Recombinant: 别称

IGF-1 Protein, Human; IGF-I Protein, Human; IGF1A Protein, Human; IGFI Protein, Human; IGF‑I Protein, Human; MGF Protein, Human

IGF1 背景信息

IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ) and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
全称
insulin-like growth factor 1 (somatomedin C)
参考文献
  • Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611.
  • de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184.
  • le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.
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