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小鼠 ABHD13 基因ORF全长cDNA克隆(表达载体), C-OFPSpark 标签

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    小鼠 ABHD13 cDNA Clone产品信息
    NCBI注册码:NM_026868.1
    参考序列ORF长度:1014bp
    cDNA基因描述:Full length Clone DNA of Mus musculus abhydrolase domain containing 13 with C terminal OFPSpark / RFP tag.
    分子别称:AI463703, AI788994, 1110065L07Rik
    分子种属:Mouse
    载体:pCMV3-C-OFPSpark
    质粒:
    限制性酶切位点:
    Tag序列:OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
    序列信息:
    测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with ABHD13 qPCR primers for gene expression analysis, MP201547 )
    启动子:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    抗生素(大肠杆菌):Kanamycin
    抗生素(哺乳动物细胞):Hygromycin
    运输方式:Each tube contains lyophilized plasmid.
    储存方法:The lyophilized plasmid can be stored at room temperature for three months.
    OFP (OFPSpark) / RFP Tag Info

    OFPSpark is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark is more than twice brighter than mOrange2. Fast OFPSpark maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection. OFPSpark can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark performs well in some fusions and protein labeling applications.

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    货号: MG51674-ACR
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