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小鼠 MCP-1/CCL2 基因ORF全长cDNA克隆(表达载体), C-Flag 标签

  • Human MDK / NEGF2 transcript variant 2 ORF mammalian expression plasmid, C-Flag tag
  • Mouse CCL2 / MCP-1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
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小鼠 CCL2 cDNA Clone产品信息
NCBI注册码:NM_011333.3
参考序列ORF长度:447bp
cDNA基因描述:Full length Clone DNA of Mus musculus chemokine (C-C motif) ligand 2 with C terminal Flag tag.
分子别称:JE, HC11, MCAF, MCP1, MCP-1, Scya2, Sigje, SMC-CF, AI323594, Ccl2
分子种属:Mouse
载体:pCMV3-C-FLAG
质粒:pCMV3-mCCL2-Flag
限制性酶切位点:KpnI + XbaI (6kb + 0.5kb)
Tag序列:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
序列信息:Identical with the Gene Bank Ref. ID sequence.
测序引物:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with CCL2 qPCR primers for gene expression analysis, MP200388 )
启动子:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
抗生素(大肠杆菌):Kanamycin
抗生素(哺乳动物细胞):Hygromycin
运输方式:Each tube contains lyophilized plasmid.
储存方法:The lyophilized plasmid can be stored at room temperature for three months.
小鼠 CCL2 基因质粒图
Mouse CCL2 / MCP-1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Product nameProduct name
研究背景

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection [7–9]. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

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货号: MG50368-CF
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Datasheet & Documentation

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