Text Size:AAA

小鼠 MBL-2 / MBL HEK293 过表达细胞裂解液 (阳性对照)

    产品数据评论相关产品实验方法
    小鼠 Mbl2 细胞裂解液产品信息
    表达宿主:Human Cells
    产品介绍:Human Cell lysate that Mouse MBL-2 / MBL transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
    序列信息:A DNA sequence encoding the mouse Mbl2 (NP_034906.1) (Glu19-Asp244) was expressed with the Fc region of mouse IgG1 at the N-terminus.
    预测N端:Asp
    分子量:The recombinant mouse Mbl2 consists 462 amino acids and predicts a molecular mass of 50.6 kDa.
    分子种属:Mouse
    小鼠 Mbl2 细胞裂解液使用指导
    制备方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
    裂解缓冲液:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
    质控:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
    稳定性:Samples are stable for up to twelve months from date of receipt.
    使用建议:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
    储存缓冲液:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
    储存方法:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
    应用小提示:Western blot (WB): Use at an assay dependent dilution.
    Other Applications: Not tested.
    Optimal dilutions/concentrations should be determined by the end user.
    MBL/MBL-2 研究背景

    MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.

    小鼠 MBL/MBL-2 参考资料
  • Garred P, et al. (2006) Mannose-binding lectin and its genetic variants. Genes Immun. 7(2): 85-94.
  • Brown KS, et al. (2007) Mannan binding lectin and viral hepatitis. Immunol Lett. 108(1): 34-44.
  • Garred P. (2008) Mannose-binding lectin genetics: from A to Z. Biochem Soc Trans. 36(Pt 6): 1461-6.
  • Garred P, et al. (2009) MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement. Mol Immunol. 46(14): 2737-44.
  • Muller YL, et al. (2010) Functional Variants in MBL2 Are Associated With Type 2 Diabetes and Pre-Diabetes Traits in Pima Indians and the Old Order Amish. Diabetes. 59(8): 2080-5.
  • Size / Price
    货号: 50063-M04HL-300
    目录价: 
    单价:      (You Save: )

    Datasheet & Documentation

    Contact Us
      您曾浏览过的产品
        产品数据可能随批次变化,最新数据请详见随货说明书。
        请注意:所有产品都是“仅用于科研,而不能用于诊断或治疗用途”