PARP Protein, Mouse, Recombinant (His Tag)

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PARP Protein, Mouse, Recombinant (His Tag): 产品信息

纯度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind  biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.
蛋白构建
A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.
NP No.
表达宿主
Baculovirus-Insect Cells
种属
Mouse
预测 N 端
Met
分子量
The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 0.1mM TCEP
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PARP Protein, Mouse, Recombinant (His Tag): 图片

PARP Protein, Mouse, Recombinant (His Tag): 别称

5830444G22Rik Protein, Mouse; Adprp Protein, Mouse; Adprt1 Protein, Mouse; AI893648 Protein, Mouse; ARTD1 Protein, Mouse; C80510 Protein, Mouse; PARP Protein, Mouse; parp-1 Protein, Mouse; PPOL Protein, Mouse; sPARP-1 Protein, Mouse

PARP 背景信息

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
全称
poly (ADP-ribose) polymerase 1
参考文献
  • Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
  • Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
  • Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
  • Ahell I. et al., 2008, Nature. 451: 81-85.
  • Structure-based mechanism of action of a viral poly (ADP-ribose) polymerase 1-interacting protein facilitating virus replication
    Author
    Chung, WC;Kim, J;Kim, BC;Kang, HR;Son, J;Ki, H;
    Year
    2018
    Journal
    IUCrJ
    Application
    SPR
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