Anti-S100A4 Antibody

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Anti-S100A4 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-S100A4 Antibody
经验证的应用
WB,ELISA
交叉反应
Reacts with: Mouse
特异性
Mouse S100A4
免疫原
Recombinant Mouse S100A4 protein (Catalog#50484-M07E)
制备方法
Produced in rabbits immunized with purified, recombinant Mouse S100A4 (rM S100A4 ; Catalog#50484-M07E; NP_035441.1; Met1-Lys101). S100A4 specific IgG was purified by Mouse S100A4 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-S100A4 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-S100A4 Antibody (Rabbit Polyclonal 抗体) 图片

Anti-S100A4 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: NIH-3T3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:10 kDa

(We are unsure as to the identity of these extra bands.)

Anti-S100A4 Antibody: 别称

Anti-18A2 Antibody; Anti-42a Antibody; Anti-Capl Antibody; Anti-FSp1 Antibody; Anti-metastasin Antibody; Anti-Mts1 Antibody; Anti-PeL98 Antibody; Anti-pk9a Antibody

S100A4 背景信息

S1A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S1 proteins containing two EF-hand calcium-binding motifs. In humans at least 2 S1 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S1A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S1A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S1A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
全称
S100 calcium binding protein A4
参考文献
  • Ambartsumian N. et al., 1995, Gene. 159: 125-30.
  • Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22.
  • Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8.
  • Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30.
  • Boye K. et al., 2008, Int J Cancer. 123: 1301-10.
  • Garrett SC. et al., 2006, J Biol Chem. 281: 677-80.
  • Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
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