SCF Protein, Human, Recombinant (aa 1-189, His Tag)

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SCF Protein, Human, Recombinant (aa 1-189, His Tag): 产品信息

纯度
> 92 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is 2-8 ng/mL.
蛋白构建
A DNA sequence encoding the amino acid (Met 1-Ala 189) of human SCF (P21583-1) extracellular domain was expressed, with a polyhistidine tag at the C-terminus.
NCBI Reference No.
表达宿主
Baculovirus-Insect Cells
种属
Human
预测 N 端
Glu 26
分子量
The secreted recombinant human SCF (aa 1-189) consists of 175 amino acids and predicts a molecular mass of 19.9 kDa. The apparent molecular mass of rh SCF is approximately 22 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SCF Protein, Human, Recombinant (aa 1-189, His Tag): 图片

SCF Protein, Human, Recombinant (aa 1-189, His Tag): 别称

C-kit ligand Protein, Human; DCUA Protein, Human; FPH2 Protein, Human; FPHH Protein, Human; Kitl Protein, Human; KL-1 Protein, Human; MGF Protein, Human; SCF Protein, Human; SF Protein, Human; SHEP7 Protein, Human

SCF 背景信息

Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
全称
KIT ligand
参考文献
  • McNiece IK, et al. (1995) Stem cell factor. J Leukoc Biol. 58(1): 14-22.
  • Besmer P, et al. (1993) The kit-ligand (steel factor) and its receptor c-kit/W: pleiotropic roles in gametogenesis and melanogenesis. Dev Suppl. 1993:125-37.
  • Mekori YA, et al. (1993) IL-3-dependent murine mast cells undergo apoptosis on removal of IL-3. Prevention of apoptosis by c-kit ligand. J Immunol. 151(7): 3775-84.
  • Automating hESC differentiation with 3D printing and legacy liquid handling solutions
    Author
    Zluhan, E;Kelly, K;LeClair, N;Wortel, D;Moody, K;
    Year
    2016
    Journal
    MethodsX
    Application
    culture
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