Anti-Cyclophilin A Antibody

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Anti-Cyclophilin A Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Cyclophilin A Antibody
经验证的应用
WB,IHC-P,IP
交叉反应
Reacts with: Human
特异性
Human Cyclophilin A
免疫原
E. coli-derived Human Cyclophilin A/PPIA fragment
制备方法
Produced in rabbits immunized with E. coli-derived Human Cyclophilin A/PPIA fragment, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
PBS, pH7.0 with 0.03% Proclin300
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-Cyclophilin A Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:2000-1:10000
IHC-P 1:200-1:1000
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cyclophilin A Antibody (Rabbit Polyclonal 抗体) 图片

Anti-PPIA rabbit polyclonal antibody at 1:5000 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: HepG2 Whole Cell Lysate

Lane D: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:18 kDa

Immunochemical staining of human PPIA in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

PPIA was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-PPIA rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PPIA rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :17 kDa

Anti-Cyclophilin A Antibody: 别称

Anti-CYPA Antibody; Anti-CYPH Antibody; Anti-HEL-S-69p Antibody

Cyclophilin A 背景信息

Peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, Rotamase A, Cyclophilin A, Cyclosporin A-binding protein, PPIA and CYPA, is a cytoplasm protein which belongs to thecyclophilin-type PPIase family and PPIase A subfamily. Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity, suggesting that they influence the conformation of proteins in cells. PPIA / Cyclophilin A accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA / Cyclophilin A is secreted by vascular smooth muscle cells in response to inflammatory stimuli, and could thus contribute to atherosclerosis. It is not essential for mammalian cell viability. PPIA / Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.
全称
peptidylprolyl isomerase A (cyclophilin A)
参考文献
  • Haendler B., et al.,(1987), Complementary DNA for human T-cell cyclophilin. EMBO J. 6:947-950.
  • Haendler B., et al., (1990), Characterization of the human cyclophilin gene and of related processed pseudogenes.Eur. J. Biochem. 190:477-482.
  • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.
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