Anti-PD-1 Antibody

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Anti-PD-1 Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-PD-1 Antibody
经验证的应用
FCM
交叉反应
Reacts with: Rhesus
特异性
Rhesus PD-1
免疫原
Recombinant Rhesus PD-1/PDCD1 Protein (Catalog#90305-K08H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus PD-1/PDCD1 ( Catalog#90305-K08H; NP_001107830.1; Met1-Gln167). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #35
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PD-1 Antibody (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PD-1 Antibody (Mouse Monoclonal 抗体) 图片

Flow cytometric analysis of rhesus PDCD1 expression on rhesus blood lymphocytes. Cells were stained with purified anti-rhesus PDCD1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of lymphocytes.

PD-1 背景信息

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
全称
programmed cell death 1
参考文献
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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