Anti-BirA Antibody, Rabbit Polyclonal

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Anti-BirA Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-BirA Antibody, Rabbit Polyclonal
经验证的应用
ELISA
应用说明
(Antibody's applications have not been validated with corresponding E. coli. Optimal concentrations/dilutions should be determined by the end user.)
交叉反应
Reacts with: E. coli
特异性
E. coli BirA
免疫原
Recombinant E. coli BirA / Bifunctional protein BirA protein (Catalog#11582-E10E)
制备方法
Produced in rabbits immunized with purified, recombinant E. coli BirA / Bifunctional protein BirA (Catalog#11582-E10E; P06709; Met1-Lys321). Total IgG was purified by Protein A affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-BirA Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BirA Antibody, Rabbit Polyclonal: 别称

Anti-bioR Antibody; Anti-BirA Antibody; Anti-dhbB Antibody

BirA 背景信息

The enzyme BirA is a key reagent because of its ability to biotinylate proteins at a specific residue in a recognition sequence. We have used this enzyme to biotinylate the C termini of membrane proteins, allowing these proteins to be tetramerized by binding to streptavidin. Because of the specificity of the biotinylation at the C terminus, the orientation of the membrane proteins on the streptavidin is equivalent to that of the native protein on the cell surface. These tetrameric proteins can be used to study protein receptor-ligand interactions at the cell surface, and site-specific biotinylation can be used to study proteins in vitro using a defined orientation. The biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin. The N-terminal BirA domain is required for both transcriptional regulation of biotin synthesis and biotin protein ligase activity.
  • LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome
    Author
    Bozal-Basterra, L;Gonzalez-Santamarta, M;Muratore, V;Bermejo-Arteagabeitia, A;Da Fonseca, C;Barroso-Gomila, O;Azkargorta, M;Iloro, I;Pampliega, O;Andrade, R;Martín-Martín, N;Branon, TC;Ting, AY;Rodríguez, JA;Carracedo, A;Elortza, F;Sutherland, JD;Barrio, R;
    Year
    2020
    Journal
    Elife
    Application
    WB
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