PDCD1 was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg Hela Whole Cell Lysate
4 µL anti-PDCD1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-PDCD1 rabbit polyclonal antibody,at 1:100 dilution
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size :31 kDa