Anti-CD14 Antibody, Rabbit Polyclonal

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Anti-CD14 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-CD14 Antibody, Rabbit Polyclonal
经验证的应用
ICC/IF
交叉反应
Reacts with: Human
特异性
Human CD14
免疫原
E. coli-derived Human CD14 fragment
制备方法
Produced in rabbits immunized with E. coli-derived Human CD14 fragment, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
PBS, pH7.0 with 0.03% Proclin300
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-CD14 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD14 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of CD14 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD14 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Anti-CD14 Antibody, Rabbit Polyclonal: 别称

Anti-CD14 antigen Antibody; Anti-monocyte differentiation antigen CD14 Antibody

CD14 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
全称
CD14 molecule
Related Pathways
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.
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