MERS-CoV Nucleocapsid Antibody, Mouse MAb

RRID Number: AB_2860453
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MERS-CoV Nucleocapsid Antibody, Mouse MAb 产品信息

产品名称
MERS-CoV Nucleocapsid Antibody, Mouse MAb
经验证的应用
WB,ELISA
应用说明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特异性
MERS-CoV Coronavirus Nucleocapsid
免疫原
Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #10
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV Nucleocapsid Antibody, Mouse MAb 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV Nucleocapsid Antibody, Mouse MAb 图片

Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000

Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP

Lane A: 20ng

Lane B: 5ng

Lane C: 2ng

Secondary

Rabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.

Performed under reducing conditions.

MERS-CoV Nucleocapsid Antibody, Mouse MAb: 别称

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景信息

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV
    Author
    Milewska, A;Chi, Y;Szczepanski, A;Barreto-Duran, E;
    Year
    2020
    Journal
    bioRxiv
    Application
    IHC
  • Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV
    Author
    Okba, NMA;Widjaja, I;van Dieren, B;Aebischer, A;van Amerongen, G;de Waal, L;Stittelaar, KJ;Schipper, D;Martina, B;van den Brand, JMA;Beer, M;Bosch, BJ;Haagmans, BL;
    Year
    2020
    Journal
    Emerg Microbes Infect
    Application
    Plaque reduction neutralization assay
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