SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb

COVID-19 Nucleocapsid Research.
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SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal 抗体) 产品信息

产品名称
SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb
经验证的应用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
免疫原
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #05
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal 抗体) 图片

Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng)

Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb: 别称

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景信息

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
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