SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified

COVID-19 Spike Research
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SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified 产品信息

产品名称
SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified
经验证的应用
WB,ELISA
应用说明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特异性
SARS-CoV-2 Coronavirus spike
Has cross-reactivity in ELISA and WB with
SARS-CoV Spike S1 (Cat# 40150-V08B1)
SARS-CoV Spike RBD (Cat# 40150-V08B2)
SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H)
免疫原
Recombinant SARS-CoV-2 / 2019-nCoV Spike Protein (Catalog#40589-V08B1)
制备方法
Produced in rabbits immunized with purified, recombinant SARS-CoV-2 / 2019-nCoV Spike Protein (S1+S2 ECD)(Catalog#40589-V08B1; YP_009724390.1; Val16-Pro1213). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Spike affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified 经验证的应用

应用 推荐稀释比/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified 图片

Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.

Lane A:  SARS-CoV Spike S1 (Cat# 40150-V08B1) (5ng)

Lane B:  SARS-CoV Spike RBD (Cat# 40150-V08B2) (5ng)

Lane C:  SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H) (5ng)

Lane D:  SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V08B) (5ng)

Lane E:  SARS-CoV-2 (2019-nCoV) Spike(S1+S2 ECD) (Cat# 40589-V08B1) (5ng)

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP  at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Coronavirus spike 背景信息

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
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