SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab

RRID Number: AB_2857935
COVID-19 Spike RBD Research
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SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab 产品信息

产品名称
SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab
经验证的应用
ELISA,Neutralization
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
2019-nCoV Coronavirus spike
Has cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S1 Protein (Catalog#40591-V08B1)
No cross-reactivity in ELISA with
SARS-CoV Spike S1-mFc Protein (Catalog#40150-V08B1)
SARS-CoV Spike RBD-His Protein (Catalog#40150-V08B2)
MERS-CoV Spike S1 Protein (Cat# 40069-V08B1)
HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H)
HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H)
HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H)
HCoV-229E Spike S1 Protein (Cat# 40601-V08H)
HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B)
免疫原
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H; YP_009724390.1; Arg319-Phe541). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #57
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
内毒素
< 3 EU/mg
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab 经验证的应用

应用 推荐稀释比/用量
ELISA 0.5-1 μg/mL
Notes
Microneutralizaiton (MN): The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.41 µg/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab 图片

Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC50 is typically 3.694 nM.
The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.41 µg/mL.

SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse Mab: 别称

Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-NCP-CoV RBD Antibody; Anti-NCP-CoV s1 Antibody; Anti-NCP-CoV s2 Antibody; Anti-NCP-CoV Spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-S2 Antibody; Anti-Spike RBD Antibody

Coronavirus spike 背景信息

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
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