Anti-EpCAM Antibody, Rabbit Polyclonal

Anti-EpCAM Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-EpCAM Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IHC-P,IP
交叉反应
Reacts with: Cynomolgus
特异性
Cynomolgus EpCAM
免疫原
Recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 protein (Catalog#90299-CCCH)
制备方法
Produced in rabbits immunized with purified, recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 (Catalog#90299-CCCH; EHH55554.1; Met1-Lys265). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Cynomolgus EpCAM / TROP-1 / TACSTD1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EpCAM Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EpCAM Antibody, Rabbit Polyclonal 图片

Immunochemical staining of cynomolgus EPCAM in cynomolgus intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunochemical staining of cynomolgus EPCAM in cynomolgus skin with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

cyno EPCAM was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-cyno EPCAM rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-cyno EPCAM rabbit polyclonal antibody,at 1:330 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Anti-EPCAM rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

EpCAM 背景信息

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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