Recombinant Anti-p53 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-p53 Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-p53 Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Cynomolgus
特异性
Cynomolgus p53
免疫原
Recombinant Cynomolgus p53 / TP53 protein (Catalog#90001-CNAE)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus p53 / TP53 (Catalog#90001-CNAE; E3U906; Met1-Asp393).
来源
Monoclonal Rabbit IgG Clone #014
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-p53 Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-p53 Antibody, Rabbit Monoclonal 图片

cyno p53 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

0.5 µL anti-cyno p53 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-cyno p53 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size: 55 kDa

Anti-p53 rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:53 kDa

Observed band size:53 kDa

Recombinant Anti-p53 Antibody, Rabbit Monoclonal: 别称

Anti-p53 Antibody; Anti-TP53 Antibody

p53 背景信息

p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.
全称
tumor protein p53
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • p53 Pathway
    p53 Pathway
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway
参考文献
  • Bakhrat A, et al. (2010) Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. Apoptosis. 15(12):1425-34.
  • Kurzhals RL, et al. (2011) Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss. PLoS Genet. 7(6):e1002103.
  • Pardi N, et al. (2011) In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. Acta Biol Hung. 62(4):397-412.
  • Wells BS, et al. (2012) Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev Biol. 361(2):263-76.
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