cyno p53 was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate0.5 µL anti-cyno p53 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:
Anti-cyno p53 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 55 kDa
Observed band size: 55 kDa
Anti-p53 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:53 kDa
Observed band size:53 kDa