Anti-E-tag Antibody, Rabbit Polyclonal

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Anti-E-tag Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-E-tag Antibody, Rabbit Polyclonal
经验证的应用
WB,ICC/IF,IP
特异性
E-tag
免疫原
A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR).
制备方法
Produced in rabbits immunized with A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR), and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-E-tag Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 0.5-2 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E-tag Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-E-Tag polyclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

E-tag was immunoprecipitated using:

Lane A:0.5 mg E tag-1M-125D-His© Whole Cell Lysate

Lane B:0.5 mg His-E tag-1M-125D© Whole Cell Lysate

Lane C:0.5 mg His-1M-125D-E tag© Whole Cell Lysate

Lane D:0.5 mg E tag-1M-244P-His© Whole Cell Lysate

Lane E:0.5 mg Psetp2 Whole Cell Lysate

2 µL anti-E-tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-E-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Anti-E Tag rabbit polyclonal antibody at 1:1000 dilution.

Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug

Lane B: his-Etag-GFER(C) transfected 293 cell lysate at 2ug

Lane C: his-GFER-Etag(C) transfected 293 cell lysate at 2ug

Lane D: Etag-GSTT2h(C) transfected 293 cell lysate at 10ug

Lane E: his-GSTT2-Etag(C) transfected 293 cell lysate at 10ug

Lane F: his-Etag-GSTT2(C) transfected 293 cell lysate at 20ug

Lane G: Nagetive control at 10ug

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

E-tag 背景信息

The E Tag is a 13-peptide epitope commonly fused to the N- or C- termini of recombinantly expressed proteins. The E Tag (amino acid sequence: GAPVPYPDPLEPR) is a popular epitope tag that can be engineered to a protein through recombinant DNA technology. The E tag contains 13 amino acids and has a molecular weight of 1.4 kDa. Antibodies to the E-tag may be a useful tool in the detection and investigation of E-tagged proteins. Anti-E Tag antibodies facilitate the detection, isolation, and purification of E-tagged target proteins without a protein-specific antibody or probe. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
参考文献
  • Mout R, et al. (2017) A general method for intracellular protein delivery through 'e-tag' protein engineering and arginine functionalized gold nanoparticles. Bio Protoc 7 (24): e2661.
  • Mohammadi M, et al. (2016) Insilico analysis of three different tag polypeptides with dual roles in scfv antibodies. J Theor Biol 402 100-106.
  • Leotoing L, et al. (2011) A20-binding inhibitor of nuclear factor-kappab (nf-kappab)-2 (abin-2) is an activator of inhibitor of nf-kappab (ikappab) kinase alpha (ikkalpha)-mediated nf-kappab transcriptional activity. J Biol Chem 286 (37): 32277-32288.
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