Anti-TNF-alpha Antibody, Mouse Monoclonal

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Anti-TNF-alpha Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-TNF-alpha Antibody, Mouse Monoclonal
经验证的应用
ELISA,ELISA(Det)
交叉反应
Reacts with: Ferret
特异性
Ferret TNF-alpha
Has cross-reactivity in ELISA with
Canine TNFα
No cross-reactivity in ELISA with
Human TNFα
Mouse TNFα
E.coli cell lysate
免疫原
Recombinant Ferret TNFA / TNF-alpha protein (Catalog#60002-FNAE)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret TNFA / TNF-alpha (rF TNFA / TNF-alpha; Catalog#60002-FNAE; A3FBF1; Val 77-Leu 233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #11
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-TNF-alpha Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ELISA 1:1000-1:2000
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Ferret TNF-alpha / TNFA / TNFSF2 in ELISA pair set (Catalog: # SEK60002). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 60002-R303).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

TNF-alpha 背景信息

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

全称
tumor necrosis factor
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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