Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal

RRID Number: AB_2860433
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Aequorea victoria
特异性
Aequorea victoria GFP Tag
免疫原
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
来源
Monoclonal Rabbit IgG Clone #208
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1/1000-1/5000
ELISA 1/10000-1/20000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-GFP Tag Antibody, Rabbit Monoclonal 图片

Anti-GFP Tag was immunoprecipitated using:

Lane A: GFP transfected E.coli cell lysate (0.5mg)

Lane B: GFP transfected 293 cell lysate (0.5mg)

 

Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GFP rabbit monoclonal antibody at 1:500 dilution

Lane A: GFP transfected E.coli lysate (5ug)

Lane B: GFP transfected 293 Cell lysate (30ug)

Lane C: Non-transfected 293 Cells lysate (30ug)

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

GFP Tag 背景信息

Green fluorescent protein (GFP tag) is a ~27 kDa protein consisting of 238 amino acids derived from jellyfish Aequorea Victoria. GFP exhibits bright green fluorescent light when exposed to blue light. It has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. Anti GFP-tag antibodies are often used in biological research and related fields. GFP tag antibodies are also often been used to study the expression of proteins and the interaction between proteins and proteins.
参考文献
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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