Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb

RRID Number: AB_1960277

Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb 产品信息

Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
H5N1 Hemagglutinin/HA
Has cross-reactivity in ELISA with
H5N1 (A/turkey/Turkey/1/2005) HA
H5N1 (A/chicken/India/NIV33487/2006) HA
H5N1 (A/Hong Kong/483/97) HA
H5N1 (A/whooper swan/mongolia/244/2005) HA
H5N1 (A/Viet nam/1194/2004) HA
H5N1 (A/goose/Guiyang/337/2006) HA
H5N1 (A/Indonesia/5/2005) HA
H5N1 (A/Cambodia/R0405050/2007) HA
H5N1 (A/duck/Hunan/795/2002) HA
H5N1 (A/Egypt/2321-NAMRU3/2007) HA
H5N1 (A/Egypt/N05056/2009) HA
H5N3 (A/duck/Hokkaido/167/2007) HA
H5N8 (A/duck/NY/191255-59/2002) HA
H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
H5N1 (A/Common magpie/Hong Kong/2256/2006) HA
No cross-reactivity in ELISA with
H1N1 (A/California/04/2009) HA
H1N1 (A/Brisbane/59/2007) HA
H3N2 (A/Brisbane/10/2007) HA
Influenza B (B/Florida/4/2006) HA
Human cell lysate (293 cell line)
Recombinant H5N1 HA protein
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H5N1 (Avian Flu) hemagglutinin extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #9F2E3F3
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb 经验证的应用

应用 推荐稀释比/用量
WB 0.1-1 μg/mL
ELISA 0.5-1 μg/mL
Block: This antibody has neutralizing activity against H5N1 (A/Anhui/1/2005) strain and H5N1 (A/bar-headed/goose/Qinghai/14/2008) strain, but no neutralizing activity against H1N1 strains through pseudotyped neutralization assay. Other H5N1 strains were not tested. The concentration of this antibody with 50% neutralization is 2 μg/ml.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin/HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
  • Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen.
    K Uranowska, J Tyborowska, A Jurek…
    Acta biochimica polonica
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