Influenza B Hemagglutinin / HA Antibody, Rabbit MAb

EliteRmab®, manufactured by recombinant technology

Influenza B Hemagglutinin / HA Antibody, Rabbit MAb 产品信息

Influenza B Hemagglutinin / HA Antibody, Rabbit MAb
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Influenza B Hemagglutinin/HA
Has cross-reactivity in ELISA and WB with
Influenza B (B/Brisbane/60/2008) HA
Influenza B (B/Colorado/06/2017) HA
Influenza B (B/PHUKET/3073/2013) HA
Influenza B (B/Washington/02/2019) HA

Has cross-reactivity in ELISA with
H1N1 (A/California/07/2009) HA

Medium/Minimal cross-reactivity in ELISA with
H3N2 (A/Hong Kong/2671/2019) HA
No cross-reactivity in ELISA and WB with
H1N1 (A/Brisbane/02/2018) HA
H1N1 (A/California/04/2009) HA
H1N1 (A/California/07/2009) HA
H1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HA
H1N1 (A/Michigan/45/2015) HA
H1N1 (A/Puerto Rico/8/1934) HA
H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA
H3N2 (A/Brisbane/10/2007) HA
H3N2 (A/Cambodia/e0826360/2020) HA
H3N2 (A/Hong Kong/45/2019) HA
H3N2 (A/Hong Kong/4801/2014) HA
H3N2 (A/Kansas/14/2017) HA
H3N2 (A/Singapore/INFIMH-16-0019/2016) HA
H3N2 (A/Switzerland/9715293/2013) HA

No cross-reactivity in ELISA with
H2N2 (A/Canada/720/2005) HA
H4N6 (A/mallard/Ohio/657/2002) HA
H5N1 (A/Anhui/1/2005) HA
H6N1 (A/northern shoveler/California/HKWF115/2007) HA
H7N7 (A/Netherlands/219/03) HA
H8N4 (A/pintail duck/Alberta/114/1979) HA
H9N2 (A/Hong Kong/1073/99) HA
H10N3 (A/duck/Hong Kong/786/1979) HA
H11N2 (A/duck/Yangzhou/906/2002) HA
H12N5 (A/green-winged teal/ALB/199/1991) HA
H13N8 (A/black-headed gull/Netherlands/1/00) HA
H15N8 (A/duck/AUS/341/1983) HA
H16N3 (A/black-headed gull/Sweden/5/99) HA

No cross-reactivity in WB with
H3N2 (A/Hong Kong/2671/2019) HA
Recombinant Influenza B virus HA protein (Catalog#11053-V08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Influenza B Hemagglutinin (Catalog#11053-V08H; ACA33493.1; Met 1-Ala 555) .
Monoclonal Rabbit IgG Clone #004
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza B Hemagglutinin / HA Antibody, Rabbit MAb 经验证的应用

应用 推荐稀释比/用量
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect influenza B virus HA in WB. Using a DAB detection system, the detection limit for influenza B virus HA is approximately 1 ng/lane under non-reducing conditions and 10 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin/HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
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