Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified

1/1
Price:
Size:
Number:

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified 产品信息

产品名称
Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified
经验证的应用
WB,ELISA
应用说明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特异性
H1N1 Hemagglutinin/HA
免疫原
Recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Protein (Catalog#11684-V08H)
制备方法
Produced in rabbits immunized with purified, recombinant Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA ( Catalog#11684-V08H; ABD77675.1; Met1-Gln528). Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA specific IgG was purified by Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified 经验证的应用

应用 推荐稀释比/用量
WB 1:2000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified 图片

Anti-Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA rabbit polyclonal antibody at 1:2000 dilution

Sample:Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin / HA Recombinant Protein

Lane A: 20ng

Lane B: 5ng

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Hemagglutinin/HA 背景信息

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
全称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
  • Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2
    Author
    Takeda, Y;Murata, T;Jamsransuren, D;Suganuma, K;Kazami, Y;Batkhuu, J;Badral, D;Ogawa, H;
    Year
    2020
    Journal
    Viruses
    Application
    Wb
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.