Anti-14-3-3 sigma Antibody (PE)

1/1
Price:
Size:
Number:

Anti-14-3-3 sigma Antibody (PE) (Mouse Monoclonal 抗体) 产品信息

产品名称
Anti-14-3-3 sigma Antibody (PE)
经验证的应用
FCM
交叉反应
Reacts with: Human
特异性
Human 14-3-3 sigma
免疫原
Recombinant Human 14-3-3 sigma / Stratifin / YWHAS Protein (Catalog#10838-HNCE)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 sigma / Stratifin / YWHAS (rh 14-3-3 sigma / Stratifin / YWHAS; Catalog#10838-HNCE; NP_006133.1; Met1-Ser248) and conjugated with PE under optimum conditions, the unreacted PE was removed.
来源
Monoclonal Mouse IgG1 Clone #02
纯化
Protein A
缓冲液
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
偶联物
PE
浓度
10 μl/Test, 0.1 mg/ml
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-14-3-3 sigma Antibody (PE) (Mouse Monoclonal 抗体) 图片

Flow cytometric analysis of Human SFN expression on HeLa cells. Cells were stained with PE-conjugated anti-Human SFN. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-14-3-3 sigma Antibody (PE): 别称

Anti-YWHAS Antibody

14-3-3 sigma 背景信息

14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
全称
stratifin
参考文献
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后