Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human ABHD14B
免疫原
Recombinant Human ABHD14B protein (Catalog#13913-H07E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; Catalog#13913-H07E; Q96IU4-1; Met1-Gln210).
来源
Monoclonal Rabbit IgG Clone #011
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal 图片

Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:23 kDa

ABHD14B was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ABHD14B rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ABHD14B rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Recombinant Anti-ABHD14B Antibody, Rabbit Monoclonal: 别称

Anti-CIB Antibody; Anti-HEL-S-299 Antibody

ABHD14B 背景信息

ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
全称
abhydrolase domain containing 14B
参考文献
  • Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.
  • Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.
  • Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
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