Anti-ACBD6 Antibody, Mouse Monoclonal

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Anti-ACBD6 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-ACBD6 Antibody, Mouse Monoclonal
经验证的应用
WB,ELISA,ELISA(Det),IP
交叉反应
Reacts with: Human
特异性
Human ACBD6
No cross-reactivity in ELISA with
Insect cell lysate
免疫原
Recombinant Human ACBD6 protein (Catalog#11112-H07B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACBD6 (rh ACBD6; Catalog#11112-H07B; NP_115736.1; Met 1-Ala 282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
来源
Monoclonal Mouse IgG1 Clone #4A9D5E9
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ACBD6 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:5000-1:10000
IP 1-2 μL/mg of lysate
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Human ACBD6 in ELISA pair set (Catalog: # SEK11112). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11112-MM07).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ACBD6 Antibody, Mouse Monoclonal 图片

ACBD6 was immunoprecipitated using:

Lane A:0.5 mg hela Whole Cell Lysate

0.5 µL anti-ACBD6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ACBD6 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 31 kDa

Observed band size: 31 kDa

Anti-ACBD6 mouse monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:35 kDa

ACBD6 背景信息

Human acyl-coenzyme A binding domain-containing member 6 (ACBD6) is a modular protein that carries an acyl-CoA binding domain at its N terminus and two ankyrin motifs at its C terminus. In mammals, there are six members of the acyl-CoA binding domain-containing (ACBD) family, and their annotation is not uniform. All six ACBD proteins contain an ACB domain at the N terminus, but they do not share significant homology at the C-terminal region. ACBD6 is a 32 kDa protein that is predicted by sequence analysis to carry an ACB domain between residues 42 and 125 and two ANK motifs at its C terminus. This protein binds long-chain acyl-CoAs with a strong preference for unsaturated, C18:1-CoA and C20:4-CoA, over saturated, C16:0-CoA, acyl species. ACBD6 is not a ubiquitous protein, but it is expressed in hematopoietic tissues and appears to be restricted to primitive stem cells present in those tissues with functions in blood and vessel development. ACBD6 was detected in bone marrow, spleen, placenta, cord blood, circulating CD34+ progenitors, and embryonic-like stem cells derived from placenta. In placenta, the protein was only detected in CD34+ progenitor cells present in blood and CD31+ endothelial cells surrounding the blood vessels. These cells were also positive for the marker CD133, and they probably constitute hemangiogenic stem cells, precursors of both blood and vessels. We propose that human ACBD6 represents a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.
全称
acyl-CoA binding domain containing 6
参考文献
  • Soupene E, et al. (2008) Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells. J Lipid Res. 49(5): 1103-12.
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