Anti-AKR1B1 Antibody, Rabbit Polyclonal

1/2
Price:
Size:
Number:

Anti-AKR1B1 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-AKR1B1 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human AKR1B1
免疫原
Recombinant Human AKR1B1 protein (Catalog#11294-H07E)
制备方法
Produced in rabbits immunized with purified, recombinant Human AKR1B1 (rh AKR1B1; Catalog#11294-H07E; P15121; Met1-Phe316). AKR1B1 specific IgG was purified by Human AKR1B1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-AKR1B1 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-AKR1B1 Antibody, Rabbit Polyclonal 图片

AKR1B1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg A431 Whole Cell Lysate

2 µL anti-AKR1B1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-AKR1B1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Anti-AKR1B1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: MCF-7 Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:34 kDa

(We are unsure as to the identity of these extra bands.)

Anti-AKR1B1 Antibody, Rabbit Polyclonal: 别称

Anti-ADR Antibody; Anti-ALDR1 Antibody; Anti-ALR2 Antibody; Anti-AR Antibody; Anti-MGC1804 Antibody

AKR1B1 背景信息

Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.
全称
aldo-keto reductase family 1, member B1 (aldose reductase)
参考文献
  • Huang SP, et al. (2010) Aldo-Keto Reductases in the Eye. Journal of Ophthalmology. 326 (3): 625-36.
  • Aida K, et al. (2000) Disruption of Aldose Reductase Gene (Akr1b1) Causes Defect in Urinary Concentrating Ability and Divalent Cation Homeostasis. Biochemical and Biophysical Research Communications.277 (2): 281-6.
  • Liao CS, et al. (2009) Regulation of AKR1B1 by thyroid hormone and its receptors. Molecular and Cellular Endocrinology. 307 (1-2): 109-17.
  • Baba SP, et al. (2009) Posttranslational glutathiolation of aldose reductase (AKR1B1): A possible mechanism of protein recovery from S-nitrosylation. Chemico-Biological Interactions. 178 (1-3): 250-8.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.