Anti-ALDH3A1 Antibody, Rabbit Polyclonal

Anti-ALDH3A1 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ALDH3A1 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IHC-P,IP
交叉反应
Reacts with: Human
特异性
Human ALDH3A1
免疫原
Recombinant Human ALDH3A1 protein (Catalog#12523-H07B)
制备方法
Produced in rabbits immunized with purified, recombinant Human ALDH3A1 (rh ALDH3A1; Catalog#12523-H07B; AAH04370.1; Met1-His453). ALDH3A1 specific IgG was purified by Human ALDH3A1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ALDH3A1 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:10000-1:20000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ALDH3A1 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of human ALDH3A1 in human stomach with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human ALDH3A1 in human lung with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

ALDH3A1 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

2 µL anti-ALDH3A1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ALDH3A1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 55 kDa

Anti-ALDH3A1 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ALDH3A1 Antibody, Rabbit Polyclonal: 别称

Anti-ALDH3 Antibody; Anti-ALDHIII Antibody

ALDH3A1 背景信息

Aldehyde dehydrogenase 3A1 (ALDH3A1) is a metabolic enzyme that catalyzes the oxidation of various aldehydes. Certain types of epithelial tissues in mammals, especially those continually exposed to environmental stress (e.g., corneal epithelium), express ALDH3A1 at high levels and its abundance in such tissues is perceived to help to maintain cellular homeostasis under conditions of oxidative stress. Metabolic as well as non-metabolic roles for ALDH3A1 have been associated with its mediated resistance to cellular oxidative stress. Aldehyde dehydrogenase 1A1 (ALDH1A1) and ALDH3A1 are corneal crystallins. They protect inner ocular tissues from ultraviolet radiation (UVR)-induced oxidative damage through catalytic and non-catalytic mechanisms. Additionally, ALDH3A1 has been postulated to play a regulatory role in the corneal epithelium based on several studies that report an inverse association between ALDH3A1 expression and corneal cell proliferation. Aldehyde dehydrogenase 3A1 (ALDH3A1) plays an important role in many cellular oxidative processes, including cancer chemoresistance, by metabolizing activated forms of oxazaphosphorine drugs such as cyclophosphamide (CP) and its analogues, such as mafosfamide (MF), ifosfamide (IFM), and 4-hydroperoxycyclophosphamide (4-HPCP). Compounds that can selectively target ALDH3A1 could permit delineation of its roles in these processes and could restore chemosensitivity in cancer cells that express this isoenzyme. ALDH3A1 may act to protect corneal cells against cellular oxidative damage by metabolizing toxic lipid peroxidation products (e.g., 4-HNE), maintaining cellular GSH levels and redox balance, and operating as an antioxidant.
全称
aldehyde dehydrogenase 3 family, member A1
参考文献
  • Pappa A, et al. (2003) Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea. Biochem J. 376(3): 615-23.
  • Estey T, et al. (2007) ALDH3A1: a corneal crystallin with diverse functions. Experimental Eye Research. 84(1):3-12.
  • Pappa A, et al. (2003) Aldh3a1 protects human corneal epithelial cells from ultraviolet- and 4-hydroxy-2-nonenal-induced oxidative damage. Free Radical Biology and Medicine. 34(9):1178-89.
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