Anti-Aminoacylase 1 Antibody, Mouse Monoclonal

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Anti-Aminoacylase 1 Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-Aminoacylase 1 Antibody, Mouse Monoclonal
经验证的应用
WB,ELISA
交叉反应
Reacts with: Human
特异性
Human Aminoacylase 1
No cross-reactivity in ELISA with
Insect cell lysate
免疫原
Recombinant Human ACY1 protein (Catalog#10549-H08B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 (rh ACY1; Catalog#10549-H08B; NP_000657.1; Met 1-Ser 408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #7A11C6C2
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Aminoacylase 1 Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Aminoacylase 1 Antibody, Mouse Monoclonal 图片

Anti-ACY1 mouse monoclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:45 kDa

Anti-Aminoacylase 1 Antibody, Mouse Monoclonal: 别称

Anti-ACY-1 Antibody; Anti-ACY1D Antibody; Anti-HEL-S-5 Antibody

Aminoacylase 1 背景信息

Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
全称
aminoacylase 1
参考文献
  • Sommer A, et al. (2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.
  • Maceyka M, et al. (2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4.
  • Cook RM, et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.
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