Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal

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Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IP
交叉反应
Reacts with: Human
特异性
Human Annexin V/ANXA5
免疫原
Recombinant Human ANXA5 protein (Catalog#10448-HNAE)
制备方法
Produced in rabbits immunized with purified, recombinant Human ANXA5 (rh ANXA5; Catalog#10448-HNAE; P08758; Met1-Asp320). ANXA5 specific IgG was purified by Human ANXA5 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal 图片

ANXA5 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-ANXA5 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ANXA5 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Anti-ANXA5 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: THP1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:35 kDa

Anti-Annexin V/ANXA5 Antibody, Rabbit Polyclonal: 别称

Anti-ANX5 Antibody; Anti-ENX2 Antibody; Anti-HEL-S-7 Antibody; Anti-PP4 Antibody; Anti-RPRGL3 Antibody

Annexin V/ANXA5 背景信息

The placental anticoagulant protein Annexin A5 (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Annexin A5 (ANXA5) is a protein abundantly expressed in normal placenta where it contributes to the healthy outcome of a pregnancy. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. ANXA5 could be used as a biomarker for the early detection of PE and for the prediction of its severity. ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. As a potential indicator for malignancy and lymphatic metastasis, ANXA5 overexpression increases in vitro migration and invasion of Hca-P cell, promotes in vivo malignancy, LNM rate and level of Hca-P-transplanted mice. Hereditary thrombophilias can impair vascular placental functions and predispose to the birth of small-for-gestational age (SGA) babies. The placental anticoagulant protein annexin A5 (ANXA5) may contribute to this process. A functional haplotype (M2) within the ANXA5 gene is associated with fetal loss and venous thrombosis.
全称
annexin A5
参考文献
  • Cederholm A, et al. (2007) Annexin A5 as a novel player in prevention of atherothrombosis in SLE and in the general population. Ann N Y Acad Sci. 1108: 96-103.
  • Schlaepfer DD, et al. (1992) Inhibition of Protein Kinase C by AnnexinⅤ. Biochemistry. 31: 1886-91.
  • Vermes I, et al. (1995) A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin Ⅴ. J Immunol Methods. 184 (1): 39-51.
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