Anti-APE1/APEX1 Antibody, Rabbit Polyclonal

Anti-APE1/APEX1 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-APE1/APEX1 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P,ICC/IF,IP
交叉反应
Reacts with: Human
特异性
Human APE1/APEX1
免疫原
A synthetic peptide corresponding to the N-terminus of the Human APEX1 / AP / APEx / Ref-1
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human APEX1 / AP / APEx / Ref-1, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-APE1/APEX1 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:1000-1:4000
ICC-IF 1:300-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-APE1/APEX1 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.
Immunochemical staining of human APEX1 in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human APEX1 in human breast with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

APEX1 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-APEX1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-APEX1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 36 kDa

Observed band size: 36 kDa

Anti-APEX1 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: HepG2 Whole Cell Lysate

Lane D: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:37 kDa

(We are unsure as to the identity of these extra bands.)

Anti-APE1/APEX1 Antibody, Rabbit Polyclonal: 别称

Anti-APE Antibody; Anti-APE1 Antibody; Anti-APEN Antibody; Anti-APEX Antibody; Anti-APX Antibody; Anti-HAP1 Antibody; Anti-REF1 Antibody

APE1/APEX1 背景信息

The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.
全称
APEX nuclease (multifunctional DNA repair enzyme) 1
参考文献
  • Ono Y, et al. (1995) Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains. Brain Res Dev Brain Res.86 (1-2): 1-6.
  • Tan Y, et al. (1996) cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues. Acta Med Okayama. 50 (1): 53-60.
  • Yao M, et al. (1999) Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1). Acta Med Okayama. 53 (6): 245-52.
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