Anti-ARIH2 Antibody, Rabbit Polyclonal

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Anti-ARIH2 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ARIH2 Antibody, Rabbit Polyclonal
经验证的应用
WB,IHC-P,ICC/IF
交叉反应
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
特异性
Human ARIH2
免疫原
A synthetic peptide corresponding to the C-terminus of the Human ARIH2
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ARIH2, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ARIH2 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
IHC-P 1:250-1:1000
ICC-IF 1:300-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ARIH2 Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of ARIH2 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ARIH2 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.
Immunochemical staining of human ARIH2 in human trachea with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).

Anti-ARIH2 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:57 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ARIH2 Antibody, Rabbit Polyclonal: 别称

Anti-ARI2 Antibody; Anti-TRIAD1 Antibody

ARIH2 背景信息

ARIH2 encodes the Triad1 protein, an anti-proliferative E3 ubiquitin ligase. In the current study, we investigate the role of Triad1 in leukemogenesis induced by an MLL1 fusion protein (Mll-Ell). we investigate the role of Triad1 in leukemogenesis induced by an MLL1 fusion protein (Mll-Ell), relevant Triad1 substrates may indicate novel therapeutic targets in this disease. E3 ligase, Ariadne homolog 2 (ARIH2), as a posttranslational negative regulator of NLRP3 inflammasome activity in macrophages. ARIH2 interacted with NLRP3 via its NACHT domain (aa 220-575) in the NLRP3 inflammasome complex. ARIH2 overexpression promoted NLRP3 ubiquitination and inhibited NLRP3 inflammasome activation. Reveal a novel mechanism of ubiquitination-dependent negative regulation of the NLRP3 inflammasome by ARIH2 and highlight ARIH2 as a potential therapeutic target for inflammatory diseases.
全称
ariadne RBR E3 ubiquitin protein ligase 2
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