Anti-ASF1A Antibody, Rabbit Polyclonal

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Anti-ASF1A Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ASF1A Antibody, Rabbit Polyclonal
经验证的应用
WB,ICC/IF,IP
交叉反应
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
特异性
Human ASF1A
免疫原
A synthetic peptide corresponding to the C-terminus of the human ASF1A
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human ASF1A, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ASF1A Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ASF1A Antibody, Rabbit Polyclonal 图片

Immunofluorescence staining of ASF1A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ASF1A polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.

ASF1A was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-ASF1A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ASF1A rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 23 kDa

Observed band size: 35 kDa

Anti-ASF1A rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:23 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ASF1A Antibody, Rabbit Polyclonal: 别称

Anti-CGI-98 Antibody; Anti-CIA Antibody; Anti-HSPC146 Antibody

ASF1A 背景信息

ASF1A has been reported to be dysregulated in multiple tumors, USP52 is a bona fide ubiquitin-specific protease, and USP52 promotes ASF1A deubiquitination and stabilization. USP52-promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression. USP52-promoted ASF1A stabilization results in growth arrest of breast cancer cells and sensitizes these cells to DNA damage. Histone chaperone, anti-silencing function-1 A (ASF1A), is crucial for post-repair H3K56Ac restoration, which in turn, is needed for the dephosphorylation of gamma-H2AX and cellular recovery from checkpoint arrest.
全称
anti-silencing function 1A histone chaperone
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