Anti-ASF1B Antibody, Mouse Monoclonal

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Anti-ASF1B Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-ASF1B Antibody, Mouse Monoclonal
经验证的应用
ICC/IF
交叉反应
Reacts with: Human
特异性
Human ASF1B
免疫原
Recombinant Human ASF1B protein (Catalog#14382-H08B)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ASF1B (rh ASF1B; Catalog#14382-H08B; Q9NVP2; Met1-Ile202). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2b Clone #02
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ASF1B Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ASF1B Antibody, Mouse Monoclonal 图片

Immunofluorescence staining of Human ASF1B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ASF1B monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nucleus.

Anti-ASF1B Antibody, Mouse Monoclonal: 别称

Anti-CIA-II Antibody

ASF1B 背景信息

The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs. This role of ASF1a in DSB repair cannot be provided by the closely related ASF1b and does not require its histone chaperone activity. Homozygous deletion of ASF1A is seen in 10%-15% of certain cancers, suggesting that loss of NHEJ may be selected in some malignancies and that the deletion can be used as a molecular biomarker for cancers susceptible to radiotherapy or to DSB-inducing chemotherapy. Anti-silencing function 1 (ASF1) is a histone H3-H4 chaperone involved in DNA replication and repair, and transcriptional regulation. Here, we identify ASF1B, the mammalian paralog to ASF1, as a proliferation-inducing histone chaperone in human β-cells. Overexpression of ASF1B led to distinct transcriptional signatures consistent with increased cellular proliferation and reduced cellular death.
全称
anti-silencing function 1B histone chaperone
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