Anti-ATF2 Antibody, Rabbit Polyclonal

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Anti-ATF2 Antibody, Rabbit Polyclonal 产品信息

产品名称
Anti-ATF2 Antibody, Rabbit Polyclonal
经验证的应用
WB,ELISA,IHC-P
交叉反应
Reacts with: Human
特异性
Human ATF2
免疫原
Recombinant Human ATF2 protein (Catalog#11599-H20B)
制备方法
Produced in rabbits immunized with purified, recombinant Human ATF2 (rh ATF2; Catalog#11599-H20B; P15336-1; Met1-Ser505). ATF2 specific IgG was purified by Human ATF2 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ATF2 Antibody, Rabbit Polyclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:5000-1:25000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ATF2 Antibody, Rabbit Polyclonal 图片

Immunochemical staining of human ATF2 in human brain with rabbit polyclonal antibody at 1:15000 dilution, formalin-fixed paraffin embedded sections.

Anti-ATF2 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:54 kDa

Observed band size:67 kDa

Anti-ATF2 Antibody, Rabbit Polyclonal: 别称

Anti-CRE-BP1 Antibody; Anti-CREB-2 Antibody; Anti-CREB2 Antibody; Anti-HB16 Antibody; Anti-TREB7 Antibody

ATF2 背景信息

Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-β signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-β stimulation. Studies indicate that ATF-2 plays a central role in TGF-β signaling by acting as a common nuclear target of both Smad and TAK1 pathways.
全称
activating transcription factor 2
Related Pathways
  • Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway
  • TNF Signaling
    TNF Signaling
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
  • IL1 signaling pathway
    IL1 signaling pathway
参考文献
  • Livingstone C, et al. (1995) ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14 (8): 1785-97.
  • Gupta S, et al. (1995) Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science . 267 (5196): 389-93.
  • Sano YJ, et al. (1999) ATF-2 Is a Common Nuclear Target of Smad and TAK1 Pathways in Transforming Growth Factor-_ Signaling. The Journal of Biological Chemistry. 274: 8949-57.
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