Anti-BID Antibody, Mouse Monoclonal

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Anti-BID Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-BID Antibody, Mouse Monoclonal
经验证的应用
WB,ELISA,ELISA(Det)
交叉反应
Reacts with: Human
特异性
Human BID
No cross-reactivity in ELISA with
E.coli cell lysate
免疫原
Recombinant Human BID protein (Catalog#10468-HNCE)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID; Catalog#10468-HNCE; P55957-1; Met 1-Asp 195). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG2a Clone #3C4C2
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-BID Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:1000-1:2000
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human p22 BID in ELISA pair set (Catalog: # SEK10468). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10468-MM10).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BID Antibody, Mouse Monoclonal 图片

Anti-BID mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

Anti-BID Antibody, Mouse Monoclonal: 别称

Anti-FP497 Antibody

BID 背景信息

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.
全称
BH3 interacting domain death agonist
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • TNF Signaling
    TNF Signaling
参考文献
  • Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.
  • Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.
  • Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.
  • Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.
  • Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.
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