Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal

RRID Number: AB_2860398
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human c-Kit
免疫原
Recombinant Human KIT / c-KIT / CD117 Protein (Catalog#11996-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human KIT / c-KIT / CD117 (rh KIT / c-KIT / CD117; Catalog#11996-H08H; P10721-2; Met1-Thr516).
来源
Monoclonal Rabbit IgG Clone #007
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal 图片

Flow cytometric analysis of Human KIT(CD117) expression on TF-1 cells. Cells were stained with purified anti-Human KIT(CD117) (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by 11996-R007 was blocked by preincubation of the purified antibody with 20ug recombinant human KIT(CD117) (Cat. No. 11996-H08H) for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of kit in HESS9 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human kit monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal: 别称

Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-PBT Antibody; Anti-SCFR Antibody

c-Kit 背景信息

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
全称
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
参考文献
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
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