Anti-C1QBP Antibody, Mouse Monoclonal

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Anti-C1QBP Antibody, Mouse Monoclonal 产品信息

产品名称
Anti-C1QBP Antibody, Mouse Monoclonal
经验证的应用
FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human C1QBP
免疫原
Recombinant Human C1QBP / HABP1 protein (Catalog#11874-H08E)
制备方法
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human C1QBP / HABP1 (rh C1QBP / HABP1; Catalog#11874-H08E; NP_001203.1; His75-Gln282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
来源
Monoclonal Mouse IgG1 Clone #09
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-C1QBP Antibody, Mouse Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-C1QBP Antibody, Mouse Monoclonal 图片

Immunofluorescence staining of Human C1QBP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human C1QBP monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human C1QBP expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human C1QBP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-C1QBP Antibody, Mouse Monoclonal: 别称

Anti-gC1Q-R Antibody; Anti-GC1QBP Antibody; Anti-gC1qR Antibody; Anti-HABP1 Antibody; Anti-p32 Antibody; Anti-SF2p32 Antibody

C1QBP 背景信息

Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner.
全称
complement component 1, q subcomponent binding protein
参考文献
  • Meenakshi J, et al. (2003) Constitutive expression of hyaluronan binding protein 1 (HABP1/p32/gC1qR) in normal fibroblast cells perturbs its growth characteristics and induces apoptosis. Biochemicaland Biophysical Research Communication. 300(3): 686-93.
  • Majumdar M, et al. (2002) Hyaluronan Binding Protein 1 (HABP1) /C1QBP/p32 Is an Endogenous Substrate for MAP Kinase and Is Translocated to the Nucleus upon Mitogenic Stimulation. Biochemical and Biophysical Research Communications. 291(4): 829-37.
  • Ghosh I, et al. (2002) Reduction in the level of hyaluronan binding protein 1 (HABP1) is associated with loss of sperm motility. Journal of Reproductive Immunology. 53(1-2): 45-54.
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