Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal

RRID Number: AB_2860230
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal
经验证的应用
ELISA(Det),FCM,ICC/IF
交叉反应
Reacts with: Human
特异性
Human CAR/CXADR
免疫原
Recombinant Human CXADR / CAR protein (Catalog#10799-H08H)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR / CAR (rh CXADR / CAR; Catalog#10799-H08H; NP_001329.1; Met1-Gly237).
来源
Monoclonal Rabbit IgG Clone #271
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human CXADR / CAR in ELISA pair set (Catalog: # SEK10799). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10799-R023).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal 图片

Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-CAR/CXADR Antibody, Rabbit Monoclonal: 别称

Anti-CAR Antibody; Anti-CAR4/6 Antibody; Anti-CXADR Antibody; Anti-HCAR Antibody

CAR/CXADR 背景信息

CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.
全称
coxsackie virus and adenovirus receptor
参考文献
  • Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352–3356.
  • van Raaij , M.J. et al., 2001, Structure. 8 (11): 1147–1155.
  • Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392–25398.
  • Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219–226.
  • Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127–131.
  • Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111.
  • Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.
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