Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology
1/2
Price:
Size:
Number:

Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal 产品信息

产品名称
Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal
经验证的应用
WB,IP
交叉反应
Reacts with: Human
特异性
Human Carbonic anhydrase II
免疫原
Recombinant Human Carbonic Anhydrase II / CA2 protein (Catalog#10478-H08E)
制备方法
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase II / CA2 (rh Carbonic Anhydrase II / CA2; Catalog#10478-H08E; NP_000058.1; Met1-Lys260).
来源
Monoclonal Rabbit IgG Clone #001
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal 经验证的应用

应用 推荐稀释比/用量
WB 1:10000-1:50000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal 图片

Anti-CA2 rabbit monoclonal antibody at 1:10000 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:30 kDa

CA2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

2 µL anti-CA2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA2 rabbit monoclonal antibody,at 1:2000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 29 kDa

Recombinant Anti-Carbonic anhydrase II Antibody, Rabbit Monoclonal: 别称

Anti-CA-II Antibody; Anti-CAC Antibody; Anti-CAII Antibody; Anti-Car2 Antibody; Anti-HEL-76 Antibody; Anti-HEL-S-282 Antibody

Carbonic anhydrase II 背景信息

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
全称
carbonic anhydrase II
参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.